Table 2. Reaction mixtures for κ-CN and β-LG gene amplification

Mixture	κ-Casein	β-Lactoglobulin
10× reaction buffer	2.5 μL (1×)	2.5 μL (1×)
dNTP	1.0 μL (100 μM)	0.8 μL (80 μM)
Primer 1	0.5 μL (1 μM)	0.3 μL (0.6 μM)
Primer 2	0.5 μL (1 μM)	0.3 μL (0.6 μM)
Taq DNA polymerase	0.4 μL (2 U)	0.3 μL (1.5 U)
dH2O	18.1 μL	18.8 μL
Genomic DNA	1.0 μL	2.0 μL
Total	25.0 μL (for one)	25.0 μL (for one)