Table 3. Thermal conditions for κ-CN and b-LG gene amplification

Reaction	Thermal condition
	κ-Casein	β-Lactoglobulin
Denaturation	95°C – 5 min	94°C – 5 min
Denaturation	95°C – 30 sec	94°C – 30 sec
Annealing	53°C – 45 sec 40 cycles	63°C – 30 sec 35 cycles
Extension	73°C – 1 min 40 cycles	72°C – 40 sec 35 cycles
Extension	73°C – 10 min	72°C – 10 min